Regulatory
RhlR+C4

Part:BBa_R0071:Design

Designed by: Ronny Krashinsky   Group: Antiquity   (2004-01-24)


Promoter (RhlR & C4-HSL regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The -10 and -35 sites are labeled as identified in [Pearson97].

The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).



Source

Pseudomonas aeruginosa rhlAB promoter

References

  • [Medina03] Medina G, Juarez K, Valderrama B, Soberon-Chavez G. Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. J Bacteriol. 2003 Oct; 185(20): 5976-83. PMID: 14526008
  • [Pearson97] Pearson JP, Pesci EC, Iglewski BH. Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J Bacteriol. 1997 Sep; 179(18): 5756-67. PMID: 9294432